International Journal of Biology Research (IJBR)

Official Journal of the Biology Research

Editor-in-Chief: Tabassum Mahboob, Director, BRC, University of Karachi

The International Journal of Biology Research (IJBR) is a bi-annual, peer-reviewed, multi-disciplinary, open access journal published by M. A. H. Qadri Biological Research Center (BRC), University of Karachi. IJBR provides publication of original research articles as well as review articles in all areas of the subject such as Anatomy, Archaeology, Biochemistry, Bioinformatics, Biomechanics, Bionomics, Biophysics, Biotechnology, Botany, Cell biology, Ecology, Entomology, Evolution, Genetics, Gerontology, Herpetology, Histology, Ichnology, Ichthyology, Immunology, Limnology, Marine biology, Microbiology, Molecular biology, Mycology, Myrmecology, Neurobiology, Ornithology, Paleontology, Palynology, Parasitology, Photobiology, Phycology, Physiology, Plant physiology, Biological psychology—Radiobiology, Sociobiology, Structural biology, Taxonomy, Virology, Zoology.

ISSN: 23109564


Muhammad Qaiser, Vice Chancellor, University of Karachi


Naimatullah Khan, Dean, Faculty of Science, University of Karachi


TabassumMahboob, Director, BRC, University of Karachi

 Associate Editors

D. Khan, ————————-

M. JavedZaki, Department of Botany, University of Karachi

S. M. Shahid, KIBGE, University of Karachi

 Managing Editors

Adnan Mujahid, BRC, Universtiy of Karachi

Muhammad Shamim, BRC, University of Karachi

 Advisory Board

 Anthony Wright, Institutionenförlaboratoriemedicin, Karolinskauniverstetssjukhuset, (Sweden)

Aqeel Ahmed, Department of Microbiology, University of Karachi (Pakistan)

Arshad Azmi, Department of Zoology, University of Karachi (Pakistan)

David Carpenter, David Axelrod Institute, Wadsworth Center, Department of Health, New York (USA)

Demosthenes B. Panagiotakos, Department of Dietetics-Nutrition, Harokopio University, Athens, (Greece)

Edith B. Allen, Department of Botany & Plant Sciences, University of California, Riverside, (USA)

FederecFumerson, INSERM U695,Faculte de Medecine Xavier Bichat, Paris, (France)

HaiderKhwaja, David Axelrod Institute, Wadsworth Center, Department of Health, New York (USA)

IsiakaOgunwande, —————— (Nigeria)

LI Yuxiu, Department of Endocrinology, Peking Union Medical College Hospital, Beijing, (China)

Liza Wong, School of Medicine and Health Sciences, University of Otago, Wellington, (New Zealand)

M. Iqbal Choudhary, HEJ Research Institute of Chemistry, University of Karachi (Pakistan)

Naji A. Mahat, Forensic Biology, Jabatan Kimia, FakultiSains, UniversitiTeknologi Malaysia,  (Malaysia)

NasiraKhatoon, Department of Zoology, University of Karachi (Pakistan)

Nessar Ahmed, School of Biology,Chemistry and Health Sciences,Manchester Metropolitan University,(UK)

Paul Brakefield, University Museum of Zoology, University of Cambridge, London, (UK)

Qamar Amin, Department of Physiology, University of Karachi (Pakistan)

RaddatzOberarzt, Abt. Gastroenterologie und Endokrinologie, UniversitätsklinikGöttingen, (Germany)

Roger D. Cox, Medical Research Council, Harwell Mammalian Genetic Unit, Oxfordshire (UK)

S. N. Hasnain, Department of Biochemistry, Dow International Medical College, Karachi (Pakistan)

SuthatFucharoen, Institute of Molecular Biosciences, Mahidol University, Salaya Campus (Thailand)

December 2015

Contents Vol. 3(2) December 2015

Noor-us-Saher, Farah Naz and Mustafa Kamal – Study on isozyme electrophoretic patterns variability in the three species of super family Grapsoidea in Karachi Mangrove Forest

M. Talha Saleem, Mehwish Amin, Ramsha Ahmed, Qudsia Basri, Sadia Ghousia Baig and Maria Baig – Comparative study of diazepam and ascorbic acid in mice by open field and balance beam test

Asma Hanif and Shahnaz Dawar – In vitro, evaluation of different concentrations of homeopathic drugs in the inhibition of root rot fungi

Sumaiya Bano, Sheeba Afsar and Jan Alam – Diversity and spatial distribution of threatened plants within Karachi University Campus

Muhammad Ajaib, Taimur Ali and Muhammad Faheem Siddiqui – A survey of ethnobotanically important herbaceous plants of Tehsil Jatoi, district Muzaffar Garh, Punjab, Pakistan

Hira Rafi and Shahnaz Dawar – Long-term resistance of bio-primed seeds in the suppression of root infecting pathogens and establishment of crop plants

Muhammad Ajaib, Musfirah Anjum, Nafeesa Zahid Malik and Muhammad Faheem Sidiqui – Ethnobotanical study of some plants of Darguti, Tehsil Khuiratta, Azad Jammu and Kashmir

Summiaya Rahim and Shahnaz Dawar – Effect of organic soil amendment on growth of plants and in the control of pathogenic fungi in Abelmoschus esculentus (L.) Moench (Okra)

Muhammad Atiqullah Khan, Shagufta Riaz and Kiran Yousuf – Size distribution and length weight relationship in commercially important fish Sillago sihama (Forsskal, 1775) (family: Sillaginidae) from Karachi coast, Pakistan

Shamsa Kanwal, Shahnaz Dawar and Marium Tariq – Impact of seed priming with Carica papaya (L.) extract in the control of root infecting fungi and growth of mash bean and chick pea (Part 1)

Shamsa Kanwal, Shahnaz Dawar and Marium Tariq – Impact of seed priming with Carica papaya (L.) extract in the control of root infecting fungi and growth of mash bean and chick pea (Part 2)

Shamsa Kanwal, Shahnaz Dawar and Marium Tariq – Impact of seed priming with Carica papaya (L.) extract in the control of root infecting fungi and growth of mash bean and chick pea (Part 3)

June 2015

Contents Vol. 3(1) June 2015

Muhammad Javed Zaki – Obituary Professor Dr. Abdul Ghaffar

Shahnaz Dawar, Marium Tariq and Farah Sultan – Fungal deterioration of cellulosic materials

Muhammad Ubaidullah Shirazi, M. Athar Khan, Shaikh M Mujtaba, Muhammad Tahir Rajput, H.B. Bozdar and Syeda Saleha Tahir – Evaluating growth and yield potential of some Brassica (Brassica juncea L. Czern.) genotypes under saline-sodic field conditions

Shahnaz Dawar, Marium Tariq, Raiha Qadri and Faiza Qismat Ali – Cereals protection from soil borne root infecting fungi by the use of homeopathic drugs

D. Khan, S. Shahid Shaukat and S. Viqar Ali – Leaf architecture and estimation of lamina area in Capparis cartilaginea Decne

D. Khan, S. Shahid Shaukat and S. Viqar Ali – Leaf architecture and estimation of lamina area in Capparis cartilaginea Decne (Table)

Ishrat Jahan, Abdul Rauf and Muhammad Anis – Effects of Wokozim organic fertilizers on germination of guar seeds

Muhammad Ajaib, Taimur Ali, Muhammad Faheem Siddiqui and Arsalan – A survey of ethnobotanically important shrubs and trees of Tehsil Jatoi, District Muzaffar Garh, Punjab, Pakistan

Muhammad Ajaib, Taimur Ali, Muhammad Faheem Siddiqui and Arsalan – A survey of ethnobotanically important shrubs and trees of Tehsil Jatoi, District Muzaffar Garh, Punjab, Pakistan (Table)

Riaz-ud-Din Qureshi and Zakia Khatoon – Biodiversity of mesozooplankton and macrofauna in marine fish ponds at Gharo, Thatta, Sindh, Pakistan

Quddusi B. Kazmi – Report of Porcellionides pruinosus (Brandt, 1833) (Isopoda, Oniscidea, Porcellionidae) from Karachi, Pakistan

Qaseem Tahera and Quddusi B. Kazmi – Occurrence of Rhopilema nomadica Galil, 1990 (Cnidaria: Scyphozoa: Rhizostomeae: Rhizostomatidae) in Pakistani waters

Nida Shaheen, Lubna Naz and Aliya Maqsood – Evaluation of clinical course and risk factors for polycystic ovary syndrome among married and unmarried women

Sulail Fatima and Lubna Naz – Differences in clinical and biochemical profiles between acute & chronic hepatitis B and C patients

Syed Ishtiaq Anjum, Karim Gabol and Abdullah – Induced toxic effects of heavy metal (chromium) on Passer domesticus (Linnaeus, 1758)

December 2014

Contents Vol. 2(2) December 2014

Farhana S. Ghory and Quddusi B. Kazmi – Developmental stages of Palaemon pacificus (Stimpson, 1860) (Crustacea, Decapoda, Palaemonidae) reared under laboratory conditions

Quddusi B. Kazmi and Moazzam Khan – Some new records of marine molluscans from the Pakistan coast

Aly Khan, Nasira Khatoon, Fatima Mujib Bilqees, Muti-ur-Rahman and Muhammad Arshad Azmi – Histopathological changes in the intestine of buzzard eagle (Butastur teesa Franklin) by acanthocephalan infection

Shahnaz Dawar, Marium Tariq and Hira Ejaz – Observation of seed borne mycoflora related with cumin (Cuminum cyminum L.)

Shahnaz Dawar, Marium Tariq and Hira Ejaz – Observation of seed borne mycoflora related with cumin (Cuminum cyminum L.) (Table)

Muhammad Tanweer Ahsan, Muhammad Arshad Azmi, Mohammad Shoaib and Imtiaz Ahmad – Toxicity of bacterial product (Abamectin), neem extract (Biosal) in comparison to synthetic pyrethroid (Lambda Cyhalothrin) in laboratory against 2nd and 3rd instars larvae of cotton leaf hopper Amrasca devastans (Distant) (Homoptera: Cicadellidae)

Muhammad Tanweer Ahsan, Imtiaz Ahmad and Mohammad Shoaib – Horizontal and vertical life tables and survivorship curves of cotton leafhopper Amrasca devastans (Distant) (Homoptera: Cicadellidae) and the distribution of mortality with age

Quratulan Ahmed, D. Khan and Farzana Yousuf – Length-weight relationship in adult Scomberomorus guttatus (Bloch & Schneider, 1801) from Karachi coast, Pakistan

Mohammad Shoaib, Syed Anser Rizvi, Aasia Karim and Muhammad Nasir – Competitive analysis of adequate fitness of nitrogen in fish ponds treated with organic and inorganic fertilizers

Naila Khatoon, Hafiza Asma Shafique, Rubina Noreen, Viqar Sultana, Jehan Ara and Syed Ehteshamul-Haque – Role of endophytic and rhizospheric fluorescent Pseudomonas associated with mungbean in suppressing the root rotting fungi of mungbeaan

Seemi Aziz and Syeda Shabi Zehra – Influence of disturbance on the seed bank dynamics of desert plants

Mohammad Shoaib, Muhammad Nasir and Aasia Kareem – Effects of ovaprim on reproductive performance of fresh water carp, Cirrhina mrigala (F. Hamilton, 1822)

Shaheen Akhter and Begum Zarina – Bitylenchus capsicumi sp.n. and one known species of the genus aphelenchus from chilli plants in Sindh, Pakistan

Muhammad Humayun Atta, Adnan Mujahid and Furqana Chaghtai – Population density of Cellana and Acmaea (Mollusca: Gastropoda) at various tidal zones on the rocky coast of Manora, Karachi, Pakistan

Safia Khanam, Javed Mustaquim and Zarrien Ayub – Study of the seasonal abundance and occurrence of macrobenthic crustaceans in mangrove swamps of Sandspit backwaters, Karachi

Abdul Hameed Solangi, Parwaiz Ahmed Baloch, Aqeel Ahmed Siddiqui, R. Uddin, Fateh Khan Nizamani and M. Zafar Iqbal – Seedling growth of three coconut (Cocos nucifera L.) varieties in Karachi, Pakistan … Short communication

June 2014

Contents Vol. 2(1) June 2014

Quddusi B. Kazmi and Razia Sultana – Updated status of Anostraca in Pakistan

Aamna Rizvi and Seemi Aziz – Population dynamics of Peristrophe paniculata (Forssk.) brummitt, a desert annual herb

D. Khan, Zulfiqar Ali Sahito and M. Javed Zaki – Axial dimensions of seeds, within-regma allocation of phytomass and seed packaging cost in a wild castor bean, Ricinus communis L. (Euphorbiaceae)

Nasira Khatoon, Aly Khan, F.M. Bilqees and Muti-ur-Rahman – Histopathology of crow (Corvus splendens Vieillot) intestine infected with acanthocephalan, Centrorhynchus amini Khan et al., 2010

Faiza A. Ansari, Shahid Hussain, Basheer Ahmed, Jameela Akhter and Erum Shoeb – Use of potato peel as cheap carbon source for the bacterial production of biosurfactants

Rakhshinda Khurram Khan, Nasira Khatoon, Rizwana, A.G. and Aly Khan – Studies on the neoplastic growth in fish Johnius dussumieri (Valenciennes, 18883) due to unknown etiology

Samrah Tariq, Hafiza Asma Shafiqe, Viqar Sultana and Syed Ehteshamul-Haque – Management of root rot disease of wheat with endophytic plant growth promoting Pseudomonas associated with healthy wheat roots

Habibullah Rana, Muhammad Farhanullah Khan, M. Faheem Akbar, Zubair Ahmed, Khalid Mahmood, Saleem Eijaz and Wali Khan – Comparative toxicological effects of Azadirachta indica extracts and cypermethrin and their effects on pupation and adult emergence against Musca domestica L.

Mursaleen Usmani, Shahid Iqbal, Hassan Fareed Siddiqui, M. Saqib Khan, Abdul Wahab, Adnan Mujahid and Muhammad Humayun Atta – Analysis of microbial quality of air in Karachi University Campus, Karachi

Tooba Khan, Zainab Sajid, Sehar Afshan Naz and Maryam Shafique – Effect of incubation period on antibacterial activity of crude fermentation broth of a guava plant associated Bacillus sp. MTZ-1 against clinical pathogens

Imtiaz Ahmad and Nasreen Khan – Aspects of sound producing organs in grylline crickets with special reference to Sciobia sp. (Orthoptera: Gryllidae) and their systematic status

December 2013

Contents Vol. 1(2) December 2013

Mahrukh Zaidi, Komal Tayyab, Ayman Wajahat, Maryam Shafique and Sehar Afshan Naz – Inhibitory effect of natural herbal extracts on systemic bacteria

Mohammad Shoaib and Muhammad Nasir – Study on the embryonic and larval development of Cirrhina mrigala f. Hamilton, 1822 in response of differential dosage of Ovaprim

D. Khan and Zulfiqar Ali Sahito – Maternal investment of biomass in pods, seeds and seed packaging in Delonix regia (Bojer) Rafin (Caesalpiniaceae)

Nasira Khatoon, Aly Khan, F.M. Bilqees, Mati-ur-Rahman And M. Arshad Azmi – Histology of the intestine of owl (Strix leptogrammica Temminck) associated with Polymorphus mohiuddini Rahman et al., 2008

Nuzzar Naseem, Muhammad Waseem Abbasi, Muhammad Javed Zaki and D. Khan – Presowing irradiance of maize caryopses with UV-C and its effects on common root-infecting fungi

Nuzhat Sultana, Iqbal Azhar and Amina Kabir – Experimental evaluation of ethanol extract of Curcuma caesia Roxb. on locomotor and learning behaviour

Zeeshan Akhtar, Ausaf Aleem, M. Aleem Ahmed, Rashida Parveen, Kamran Ahmed and Mansoor Iqbal – Screening of heterocyclic compounds: Insecticidal and fungicidal activity of substituted pyridines and their derivatives

Shahid Iqbal, Asia Neelam, Omm-e-Hany and Suraiya Jabeen – Antibacterial and antioxidant effects of chewable tobacco leaf product (Patti) and betel nut on environmental pathogens

Erum Shoeb and Maheen Waqar – Allelic variation in the genes for drug abuse and addiction: An overview

Tahir Anwar, Imtiaz Ahmad and Seema Tahir – Reporting pesticide residues in soil of Lodhran district, Punjab, Pakistan

Tahir Anwar, Imtiaz Ahmad and Seema Tahir – Reporting pesticide residues in soil of Lodhran district, Punjab, Pakistan (Table)

Mohammad Shoaib and Naveed Khan – Induction and recovery from anesthesia in Cyprinus carpio (Linnaeus, 1758) immersed in different concentrations of 2-phenoxyethanol (clove oil)

M. Humayun Atta, Adnan Mujahid and Furqana Chugtai – Distribution, abundance and population density of Cellana radiata and Acmaea sp. on the rocky coast of Buleji, Karachi, Pakistan

M. Humayun Atta, Adnan Mujahid and Furqana Chugtai – Movement of common rock clinger limpet, Cellana sp. on the rocky shore of Manora, Karachi, Pakistan

June 2013

Contents Vol. 1(1) June 2013

Muhammad Anis, M. Javed Zaki and Zeeshan Abbas – Effects of gamma irradiation on germination, growth of sunflower and control of charcoal rot fungus Macrophomina phaseolina (Tassi) Goid.

Hira Ejaz, Shahnaz Dawar, Marium Tariq and M. Javed Zaki – Survey on seed borne fungi of Punica granatum L. (Pomegranate) seeds

Hira Ejaz, Shahnaz Dawar, Marium Tariq and M. Javed Zaki – Survey on seed borne fungi of Punica granatum L. (Pomegranate) seeds (Table – 1)

Aly Khan, Nasira Khatoon, S. Shahid Shaukat and A.R. Kazmi – Nematodes associated with apple (Malus pumila (Mill.)

Sumera Sohail, Zahir Hussain, Quratul-Ain and Syed Jibran Ashraf – Blood cholesterol and leptin levels in smoking and non-smoking patients with diabetes mellitus

Junaid Mahmood Alam, Ishrat Sultana, Muhammad Zzhar Imam and Maqsood Ali Ansari – Assessment of thyroid hormones status in sub – clinical thyroid disease in selected adult patients

Nasira Khatoon, A.G. Rizwana, F.M. Bilqees, Asmatullah-Kakar and A.K. Nazia – Some cellular changes in the liver of Pomadasys olivaceus (Day) due to nematode larvae infection

Imtiaz Ahmad, Tahir Anwar and Seema Tahir – Pesticide residues in agricultural commodities in Pakistan’s perspective: with reference to safe limits

Rajput Muhammed Tariq – Asexual reproduction in hermaphroditic animals and plants, Clicks the idea of new independent sex evolution: Proved practically in the field study

Jameela Akhter, Nuzhat Ahmed, Uzma Badar, Maheen Waqar and Erum Shoeb – Heavy metal and antimicrobial resistant bacteria isolated from Karachi coastal area as an indicator of pollution

Uzma Badar, Nuzhat Ahmed and Erum Shoeb – Bioaccumulation and biosorption of copper by Pseudomonas species

Nuzhat Sultana, Rafeeq Alam Khan and Iqbal Azhar – Analgesic and anti-inflammatory activity by methanol extract of Mucuna pruriens (L.) DC

Humaira Batool, Noureen Fatima, Hira, K., Viqar Sultana, Jehan Ara and Syed Ehteshamul-Haque – Role of fluorescent Pseudomonas associated with root nodules of soybean in suppressing the root rotting fungi and root knot nematode of soybean in soil
amended with seeds of Vernonia antihelmenthica

Noreen Raza, Tasneem A. Saqib, Rajput Muhammad Tariq and Syed Naeemul Hassan Naqvi – Study of bioaccumulation of metals in tissues of Himantopus himantopus (black winged stilt) from coastal areas of Karachi, Pakistan



The International Journal of Biology Research (IJBR) accept papers that contribute to knowledge in the field of Biology and related areas. An article for publication must describe substantial original research undertaken according to the scope of this journal.

A manuscript submitted for publication implies that the work described has not been published before (except as an abstract or a published lecture, review or thesis). At the time of its submission to IJBR it should neither be under consideration for publication elsewhere nor will it be reproduced after its publication in any other journal. Any co-authors must have agreed with the contents of the publication including the institution where the work was carried out. All authors should have agreed to automatic transfer of copyright to the Editor-in-Chief.


The speed of the publication depends greatly upon following the guidelines for authors. The manuscript, carefully prepared in the style of this journal, should be submitted electronically containing a full version of the manuscript in double space on A4 size pages. Title page with author name and address, each figure, table, illustration and legends should be on separate page.

The manuscript should include an abstract, a brief introduction, materials and methods, results and discussion, acknowledgements (if any) references and 5 keywords. Addresses should include full postal address, telephone, fax number and     e-mail address of the author responsible for correspondence. Contributions are acceptable under any of the following section headings.

Original Articles describing substantial original research undertaken according to the scope of this Journal.

Short Communications consisting of brief technical views; not more than 800 words, one figure and table, and not more than six references.

Review Articles are generally invited articles.

Letters to the Editor normally be less than 400 words and be sent to the Editor-in-Chief on the matters related to published articles in the Journal or specific views within the scope of the Journal.


It should be brief indicating the contents concisely and adequately.


All figures, tables and photographs with proper titles, drawn in Microsoft word or excel should be numbered in the order in which they are mentioned

in the text. All Photographic material should be provided in “camera ready” form, trimmed to required size and format. Colored diagrams can be incorporated on payment for additional cost incurred in their printing.

The title of figures should be given at the bottom of the figures. The title of tables should be given on the top of the tables.


The references should be written in the sequence of author(s) name(s) with the surname appearing first, complete title of the article, abbreviated name of the journal in italics in proper abbreviated form, year, volume and page(s). Reference to books should be in the sequence of name(s) of author(s), title of the chapter, title of the book, edition, editor(s), publisher, city and country of publication, year and page(s). The accuracy of the reference is the responsibility of the authors. References in the text should be numbered in the sequence as these appear.

Examples of the references


Chandra AK, Medda S and Bhadra AK. Changes of activin A secretion in gestational disease. J. Ferment. Technol. 1980; 58: 1–10.


Broze Jr. GJ and Mietich JP. In: Hemostasis and Thrombosis: Basic Principles and Clinical Practice, Third Edition, Editors: Colman RW, Hirsh J, Marder VJ and Salzman EW, JB Lippincott Company, Philadelphia. 1994; pp 145-326.


Amino N, Tada H and Hidaka Y. Inherited and acquired dysfibrinogenemia. Proceedings of the 7th Asian-Pacific Congress of Clinical Biochemistry, Bangkok, Thailand, 1995; pp 165-171.


Symbols and abbreviations should be those currently used in this journal and conventionally used in the subjects of Biochemistry and Molecular Biology. Authors should not create new abbreviations and acronyms.


Any previously published data of any author should have written consent from the authors/publishers for publication in IJBR.


International Journal of Biology Research (IJBR)
M. A. H. Qadri Biological Research Center (BRC)
University of Karachi, Karachi-75270, Pakistan
Tel:         +9221-99261300-7 Ext: 2349